21 Aralık 2011 Çarşamba

How To Grow Magic Mushrooms From Spore Prints On Rice

How To Grow Magic Mushrooms From Spore Prints On Rice

The Joys Of Mushroom Gardening

More articles about mushrooms are located here.
This is one of many ways to grow mushrooms, before trying to grow them
yourself, check a few different methods to see which one suits your needs.
The original copy of this document can be found at the Lycaeum,
along with many others about all types of drugs, and other methods of growing
mushrooms.
Well, here it is, just about all I know about growing psychedelic mushrooms.
Most of this information was taken from a book entitled Magic
Mushroom Cultivation
written by Stephen H. Pollock. Anyway, I have
used the rice-cake method described below, and am currently growing my third
batch, which has produced some pretty potent mushrooms!

I feel the need to mention that I'm giving you this information for
INFORMATIONAL PURPOSES ONLY, and I don't expect you or anyone else to actually
undertake any of the techniques I will describe below, for to do so may violate
certain laws, and I wouldn't give you this information if I thought you might do
something illegal.
Before I describe the technique I use, I'd like to say that there are many
methods of growing 'shrooms, some more difficult than others, and I am simply
presenting the method which has worked well for me: I've never had a dud batch,
they've always fruited readily, and I've never poisoned myself or others with
contaminated 'shrooms.
I should mention, however, that the procedure I describe is not one which
will give maximum yields of mushrooms, but it does have the advantage that the
growing medium itself can be ingested for psychedelic effects (see section on
Storage and Methods of Ingestion).
Another point I'd like to make is that I STRONGLY recommend getting other
information on mushroom cultivation before starting your own batch. Perhaps the
best book I have seen on the subject is The
Mushroom Cultivator
by Paul Stamets. It gives extremely detailed information
on the cultivation of psychedelic mushrooms. I highly recommend that you read
this book before following any cultivation procedure.


Materials Needed To Grow Magic Mushrooms

  • A sporeprint from a strain of psychedelic mushrooms.
    (make sure it's
    the real thing, and that it's not contaminated with anything! Dust, for
    example.)
  • A pressure cooker, any size, but preferably one with 17 qt. (liquid)
    capacity.
    (this is the most expensive item, but it's a necessity. Borrow,
    rent, buy, or steal one.)
  • 12 (or more) new canning jars, 1 quart size, pref. wide mouthed, with
    lids.
  • A box/bag of brown rice -- NOT white rice. Long grain/wild rice might also
    be a good growing medium -- maybe even better than regular brown rice,
    although I'm not positive about this. I once used a half-and-half mix of brown
    rice and Long grain wild rice which worked fine. However, a possible
    disadvantage to using the long grain/wild rice is that any contaminants such
    as dark-colored molds will be more difficult to spot in the growing medium.
  • Something to scrape the spores off the print into the jar.
    You want
    something like a stiff metal wire with a handle, so you can heat the end red
    hot in a flame to sterilize it without burning your fingers. I find that a
    probe from a Biology dissection kit works wonderfully.
  • A flame source. An alcohol lamp is not hard to make out of a small jar
    filled with rubbing alcohol, with a cotton ball as a wick. I suppose you could
    just use a lighter, but i prefer making an alcohol lamp -- just make sure you
    don't burn your place down!!
  • A clean place to store your jars -- should have a relatively constant
    temp.
    (the optimum temperature for starting the 'shrooms is 86 F, but I
    have found room temperature to work fairly well). Closet shelves are fine, in
    my experience. You want a place that's pretty dust/bug free, but you don't
    want the storage area to be airtight, as shrooms do have to breathe just like
    any other living organism. Many books recommend making some kind of superclean
    box to store the jars in, but I've never bothered with that. Most sources of
    information on growing 'shrooms (this one, too) stress that everything be AS
    STERILE AS POSSIBLE. However, if you do have to cut a few corners you should
    still be successful if you just USE YOUR HEAD!
which leads me to
the....

  • optional materials: germ-killing soap for washing hands, alcohol for
    sterilizing hands, etc., surgical gloves, dust masks, hair-nets, an
    air-filtering machine (Pollenex?), a couple 1 gallon jugs of distilled water,
    a spray bottle, bleach. (As you can see, this is all stuff which will help to
    make things a bit more sterile -- definitely recommended!)


Procedure For Growing Magic Mushrooms

This is the procedure I follow for the rice-cake method of propagating
psychedelic mushrooms. I use this method for a number of reasons. One is that my
first ever batch consisted of 6 jars of manure medium and 6 of the brown rice
medium, I found the rice cakes produced more 'shrooms, and for a longer period
of time than did the manure-filled jars. Rice has obvious advantages in that
it's easy to obtain -- no trekking through a pasture looking for fresh
cow-shit!
Also, the manure stinks like hell when cooked in the pressure cooker! Perhaps
the biggest advantage to the rice cake method is that when the rice cake no
longer produces crops of 'shrooms (about 2 mos.), you can actually CONSUME THE
RICE CAKE ITSELF!! Given, of course, that you detect no contaminants on the rice
cake (molds or bacteria). When mushroom growth stops, the rice cake can provide
a trip for 2-4 people. See the end of this article for methods of ingesting
mushrooms/rice cakes.
  1. Turn off the air-conditioner in the place you're going to do this. It is
    very important to work in a draft-free area. Turning the A/C off will allow
    the dust in the room to settle (including the heavier mold spores which can
    contaminate your rice-cake medium.)
  2. Set up the pressure cooker, make sure you read the manual if you have one.
    You don't want the damn pressure cooker exploding, or anything like that. Wash
    out the pressure cooker for good measure, and also wash the jars and lids. I
    wouldn't use a towel to dry them out, though, you'll just wipe germs &
    dust back on 'em.
  3. Wash yourself, too. It's recommended that you wear a long sleeved shirt,
    and to pull your hair back or wear a cap or hair-net. I don't think that the
    dust mask would be necessary at this point, maybe later, though.
  4. For each quart-size canning jar, add 1/4 cup brown rice and 1/3 - 1/2 cup
    water.
    I use the distilled water that you can buy in any grocery store --
    I don't trust tap water. Fill 6 or 7 jars with this mixture, or as many as
    will fit into your pressure cooker without stacking or jamming them in there.
    Place the lids on the jars, with the rubber UP, and leave the lids very loose.

  5. Place the jars on the bottom rack of the pressure cooker. I recommend
    using the rack, that way the jars won't tip and spill as the water boils
    around them. Using the rack also keeps them from breaking from the heat of the
    burner directly below them. For a 17 quart pressure cooker, add about 3 quarts
    of water, but not so much that the jars start to float and tip over. Again, I
    use distilled water for this.
  6. Now, follow the directions for sealing the pressure cooker. Some recommend
    that you rub a dab of cooking oil on the seal, so that it seals properly and
    is easier to close and open. Do it right. Do it by the book. Turn the stove on
    its highest setting and allow the pressure inside the cooker to build up to 15
    lbs. Once the pressure inside the cooker has reached 15 lbs., you want to
    maintain it at that level for one complete hour. You may have to turn down the
    stove for brief periods so that the pressure doesn't rise to unsafe levels
    above 15 lbs. When the hour has passed, turn off the stove and LET THE
    PRESSURE COOKER COOL BEFORE OPENING! Also, don't try to rush the cooling
    process, as the jars may crack.
  7. Just before opening the pressure cooker, wash up again, maybe use rubbing
    alcohol or put on surgical gloves. Now is the time for dust masks (although I
    usually use my shirt to keep from breathing germs on the jars). Long sleeves
    and a hat or whatever is recommended because literally millions of germs are
    falling off your body at any given moment. Sterility and the absence of drafts
    are of utmost importance from here on out... (some books recommend filling a
    spray bottle with a 10% bleach / 90 % water solution and using it to mist the
    air in the room to further reduce airborne contaminants.)
  8. Open the pressure cooker and let the jars cool until they're pretty close
    to room temp. If you remove the jars too soon, they will crack and you will
    have to start over with new jars, so it pays to be a little patient. You may
    want to tighten the lids a bit so air/germs can't contaminate the rice cakes.
    When the jars cool off, you're ready to go.
  9. Heat your wire loop/probe/whatever until it is GLOWING RED. Put on your
    dust mask or pull your shirt up over your nose and mouth.
  10. Lift the lid off the jar and set it down on a sterile surface, with the
    inside face down. OR let a friend hold the lid for you. Make sure the person
    has washed/sterilized his/her hands as well as you have.
  11. Get out your sporeprint and hold it over the open jar at an acute angle.
    Use the sterilized wire loop/probe to gently scrape and tap the sporeprint to
    get the spores down onto the rice cake. If you can see dark specks fall onto
    the rice, you've done it sufficiently -- anything you can see is probably
    several thousand spores. A sporeprint the size of a nickel can EASILY
    inoculate a dozen jars.
  12. Screw the jar's lid on tightly and shake the jar until the rice cake
    breaks up. This will allow the spores to spread throughout the rice medium,
    thus increasing the chances for success. A good way to start the process is to
    inspect the jars carefully for cracks, invert the jar, and strike the lid
    against the heel of your hand. Next, unscrew the lid until it almost comes off
    -- this allows for air to get into the jar. I usually just screw the lid on
    about 3/4 of a turn -- just enough where it won't fall off easily.
  13. When you've done this for all your jars, put the jars in a safe, clean
    place with a fairly constant temp., a dark place is best. In 3 days - 2 weeks
    you should see white, fluffy mycelia appear -- looks like white fuzz. Any
    other color of fuzz (green, black, etc.) is mold, and the jar should be
    disposed of. I'm not kidding about this! Certain contaminants, molds in
    particular, can cause illness or even death if you ingest the contaminated
    'shrooms. It's better to be safe than sorry, believe me. Also be on the
    lookout for bacterial infections of the rice medium. These will often appear
    as colored (orange or pink) runny or clammy looking gunk in with the rice.
    These should be thrown out immediately as well. Bacterial infections may also
    give off a kind of putrid odor, but of course you should not be taking the
    lids off the jars at all during this stage. Now, the rice itself will get very
    soft as a result of the pressure cooking, and the initial shaking of the jar
    may smear gel-looking gunk all over the insides of the jar. But by comparing
    with the rest of the jars you should be able to tell the difference between
    this gunk and a bacterial infection. Like I said before, JUST USE YOUR HEAD!
  14. This is not actually another step because you're done! Just sit back and
    wait for nature to take its course! Shrooms are pretty much maintenance-free
    until fruiting starts to occur. It should take anywhere from 2 weeks to 1
    month for the mycelia to completely permeate the rice medium, then it will
    start getting these stringy looking or fan shaped runners in the white fuzzy
    growth. Mushroom formation is not far off, and the jars should be getting a
    couple of hours of light per day -- fluorescent is OK, and natural sunlight is
    superb, just make sure the jars don't get too warm. Of course at all stages be
    on the lookout for any possible contaminants in the mycelia. By the way, as
    the mycelia mature, they may start staining blue in spots, due to bruising, I
    think -- so don't mistake this for a mold infection, but keep a close eye on
    any change in color from the white coloring. The 'shrooms first appear as tiny
    white pinheads and then the caps will darken (in P. cubensis) to a
    lovely reddish brown. When the 'shrooms are growing the lids on the jars
    should be very loose to allow for air exchange.
    Also, mushrooms grow best in an environment with a humidity of over 90%, so
    if you think that your 'shrooms may need a more moist environment, one thing
    to do is to simply use a spray bottle to spray boiled or distilled water
    directly onto the lids of the jars. I find that the moisture condenses inside
    the jars and runs down the inside of the jars, moisturizing the mycelia. You
    could also VERY LIGHTLY mist the surface of the rice cake if it looks dry. You
    don't want things TOO wet, however, as this will promote mold/bacteria growth
    and actually inhibit mushroom formation. Another possible method is to replace
    the lids with a double layer of paper towel which is misted daily -- although
    I would think that not having an actual lid on the jar would invite
    contamination. Just my personal opinion. It is important that air exchange
    takes place in the storage area -- this becomes more important as fruiting
    occurs, as the mycelia gives off CO 2 and needs O 2 . Remember that CO 2 is heavier than
    normal air, so it might be good to tip the jars a few times a day to let the
    CO 2 dissipate out of the jar.



Harvesting Magic Mushrooms

'Shrooms are "ripe" as soon as the white membrane connecting the cap to the
stem has broken somewhat, although you don't want to pick them before they have
reached their full size! To harvest an individual mushroom, wash your hands
well, I use rubbing alcohol, too. Then take the lid off the jar and grasp the
mushroom firmly near the base. You may need to use a pair of sterilized tweezers
to do this, which is what I usually do, I avoid placing germy hands inside the
jars. A brisk twisting motion will help to free the 'shroom from the mycelia. If
it is too difficult to harvest them using those methods, you can clean you
hands, wash a small knife (preferably with anti-bacterial soap), dip the blade
in alcohol, flame it for several seconds, then use the tip of the sterilized
knife to cut the mushroom as close to the rice cake as possible.


Storing And Eating Magic Mushrooms

Avoid crushing fresh mushrooms before storing them. The blue staining that is
common in psychedelic mushrooms is evidence of oxidation, meaning that the
active ingredients (psilocin and psilocybin) are being oxidized, too, rendering
the 'shrooms inactive. While refrigeration is recommended, freezing fresh
mushrooms should be avoided, since the expansion of the freezing water in the
cells ruptures the cell walls and thus opens them up for oxidation. Mushrooms
that were frozen while fresh may be an attractive blue color, but they are
inactive.
Storage of fresh mushrooms should be in a breathable container such as a
paper bag stored in a refrigerator, avoid putting fresh 'shrooms in a ziploc
bag, as they may become slimy or moldy. I have heard of people also storing
fresh shrooms by chopping them up and mixing them into honey, The 'shroom honey
is then spread on bread or whatever and eaten.
There are a few methods of drying mushrooms, although I have found dried
shrooms to be MUCH weaker than fresh ones. One way to dry them is by placing
them on a cookie sheet in an oven on the lowest temp. with the door slightly
open. Simply drying them in sunlight is said to work also.
My main problem with dried shrooms is that in my experience they are not
anywhere near as potent as fresh 'shrooms. I believe the reason for this is that
the two psychoactive ingredients (psilocin and psilocybin) are present in equal
amounts in fresh shrooms. BUT, psilocin is an unstable compound compared to
psilocybin, and breaks down readily when exposed to heat and oxygen. The normal
dosage for dried shrooms is 1 - 5 grams. But I have never had a "trip" from
dried shrooms only with the fresh stuff. I ate 4 grams of dried 'shrooms once
and only got a buzz like being stoned or drunk.
So, I like my shrooms fresh, and of course, I have that luxury since I grow
my own. Whether they are dried or fresh, there are many interesting ways to
ingest them. My current favorite method is to blend 3-4 fresh ones in a blender
with orange juice, the effects are fantastic and the taste is tolerable.
I believe this is due in part to the fact that the shrooms are almost
completely liquefied by the blending process, releasing the "good stuff" into
the orange juice and making it more readily absorbed by the stomach. Some people
may say that the vitamin C in the OJ also enhances the effects, but this may be
just a myth. Another good method, one which I have used to eat the rice cakes,
was to chop the rice cake (or shrooms), and brown them for JUST a few seconds in
butter or margarine before pouring in an omelet mixture. Mushroom omelets. Not
only a meal, but a good trip, and a tasty way to ingest the shrooms, (I happen
to dislike the taste of shrooms by themselves).

Yet another method of taking shrooms is to make a milkshake in a blender, and
add the shrooms, you can make kind of a "strawberry smoothie" in this way.
Remember though, that dairy products may delay/block the absorption of certain
substances. Another method of ingestion is to boil the shrooms, fresh or dried
(or a rice cake) in a couple cups of water for about 5 minutes (until they have
sunk, one source says), and then either add a tea bag for hot tea, or make
Kool-Aid with the cooled water (straining out the shrooms, of course).
Sprinkling fresh or dried shrooms (chopped) onto pizza, or into spaghetti
sauce is another treat, fun for a "shroom party". Since psilocin and psilocybin
are soluble in both water and alcohol, soaking shrooms in any liquor will
release these active ingredients into the liquor, making for a powerfully
intoxicating liquor a la' the way an "Emerald Dragon" is made with marijuana...
I have tried smoking a couple dried shroom caps, but only got the slightest buzz
from the VERY harsh smoke, no real effects to tell the truth.
I should mention again that once shroom production has really tapered off
(and you'll be able to tell) after 2 - 3 months, the rice cake can be
eaten/used, if you closely examine it and decide that there is no green or black
mold contaminant present.
I should note that the rice cake will probably be all kinds of funky colors
-- a mix of white, steel blue, gray, maybe even purple in places from spores
falling on it! I have ingested several scary-looking rice cakes, however, with
no ill effects. Again, USE YOUR HEAD! If in doubt, toss it out -- it's not worth
a trip (no pun intended) to the hospital. A single rice cake is enough for 2 - 4
people to trip on, although 2 is probably the better figure. Some of my best
trips were on half a rice cake chopped up and cooked in an omelet!
That's what I love about the rice-cake method, when the shrooms stop growing
there's no waste. Speaking of no waste, if I ever had a rice cake that I didn't
want to risk eating I might use it to inoculate a compost pile or a pasture full
of cow shit by inserting a small piece into each cow-pie or into the compost
pile. Just think of the idea of starting a culture of wild mushrooms in your
area. :-)


Making Spore Prints Of Magic Mushrooms

This is really easy, just wash your hands well, then take a fresh shroom and
gently twist the cap off away from the stem ( OR, I usually use a sterilized
knife blade to cut the stem off as close to the cap as I can without touching it
too much). Then place the cap, gills down, on a sterile card or piece of
glass.
Cover the cap and card with a clean, small container to keep drafts from
blowing the spores away, and to prevent dust/contaminants from settling on the
card/glass. I usually use a small juice glass for this purpose. Leave the
covered 'shroom cap on the card/glass overnight and, voila! I suggest folding
the card the next day and keeping it in an airtight container (small ziploc bag)
in a refrigerator.

I have been told that spore prints will keep for up to a year in an airtight
refrigerated (not frozen) environment. From personal experience I know that they
are still viable after 3 months. Oh, by the way, try to find some use for the
'shroom cap after you've collected the spores from it, it's still psychoactive,
so I'm sure you can think of something to do with it. :-)


APPENDIX: Additional tips for more optimal yields

Here are some additional tips, based on what I have learned from The
Mushroom Cultivator
. One thing which comes to mind is that 86 F is the best
temp. for starting the growth process. Something to remember though, is that the
temp.
INSIDE the jars will be several degrees higher than the surrounding air temp.
Growth of the mycelia generates small amounts of heat. The
Mushroom Cultivator
tells all about decreasing the temperature at various
stages of growth to promote fruiting (the term they use for mushroom growth).
After reading The
Mushroom Cultivator
, I would also advise building a simple growth chamber.
This will serve a number of purposes:
  1. it will create a more sterile environment, guarding against contamination.

  2. it will help keep the temp. high and more constant.
  3. it will help keep the humidity high and more constant.
  4. it will provide a place to hide the jars, rather than just having them out
    on a shelf in your closet or wherever.
Here's what I recommend: get a Styrofoam ice chest, one that's large enough
to hold the 12 jars you've got. I used one I got at Circle K for less than $5
bucks. You may want to line the inside with aluminum foil, to increase the
reflection of light within the chamber, which will be good when you're ready to
expose the cultures to light.
At some point you'll need to cut a large hole in the lid -- cut out as much
of the lid as you can, but make sure that you leave enough of a margin on it so
that it still functions as a lid. Then use some kind of tape to tape Saran Wrap
over the hole. Now you have a lid which allows light into the grow-box, but
helps to keep out dust, mold, and other contaminants.
You don't have to mess around with the lid right away, though. The
Mushroom Cultivator
suggests leaving the jars in TOTAL darkness for the
first week or two -- even to the point that they suggest using only a red light
to examine the jars for growth and/or contaminants. The book also suggests NO
air exchanges during this initial growth phase. I guess you could leave the lids
loose like I suggested, but leave the lid on the box.
Oh, by the way, in The
Botany and Chemistry of Hallucinogens
by Schultes and Hoffman, they say that
the medium adult oral dose is 4-8 mg psilocybin. And that dried 'shrooms contain
2 to 4 percent psilocybin -- but this was from a sample of Psilocybe mexicana,
and i think P. cubensis may be more potent.

They also mention something else that's interesting. They say that psilocybin
and psilocin are present in equal amounts in fresh 'shrooms, AND that psilocin
is something like 1.4 times as psychoactive as psilocybin. Given the fact that
psilocin is sensitive to oxidation, and breaks down upon drying (i suppose),
this seems like a probable reason that fresh ones are so much more potent than
dried ones.
I have recently discovered a method of drying 'shrooms without heat, which
may help them to retain a potency level similar to fresh ones, although I
haven't tried any of the 'shrooms which I have dried by this method so I don't
know for sure that this is true. What I do is cover the bottom of a shallow
baking dish with a layer of uncooked (dry) rice, usually the rice that was left
over in the bag from the initial start-up of the procedure. Then I place a clean
paper towel over this layer of rice and place freshly harvested mushrooms on top
of the paper towel. Then I cover the dish with another clean paper towel and
place the dish in my refrigerator.
I find that the rice absorbs all the moisture from the 'shrooms, and they are
completely dried within a week. Be careful not to pile the fresh mushrooms on
top of one another in the dish, spread them out directly on the paper towel or
they may not dry, this creates the possibility of them getting moldy, which will
RUIN them! Of course, if your refrigerator is unusually humid, the 'shrooms may
take longer to dry out, if at all.
Here is something else which may be helpful. Taken without permission from
Paul Stamets' book, The
Mushroom Cultivator
. [Brackets] indicate comments from the author of this
file.


Parameters for Optimal Growth of Magic Mushrooms

[Adhere to these as much as you feel comfortable with. Like I said before,
I have obtained satisfactory results by keeping the jars on a closet shelf from
start to finish. But trying to follow these guide-lines will certainly lead to
better crop yields.]


SPAWN RUN: [1st stage of
growth]
Relative Humidity 90%
Substrate Temp. 84-86 F.
[Thermal death limits at 106 F.]
Duration 10-14 days.
CO 2 5000-10000 ppm
Fresh Air Exchanges 0 per hour.
Light Incubation in total darkness.


PRIMORDIA FORMATION: [pinhead
formation]
Relative Humidity 90+%
Air Temperature 74-78 F.
Duration 6-10 days.
CO 2 less than 5000 ppm.
Fresh Air Exchanges 1-3 per hour.
[but remember this air MUST be free of
contaminants such as dust.]
Light Diffuse natural or exposure for 12-16 hours/day of grow-lux
type fluorescent high in blue spectra at the 480 nanometer wavelength.

[I find that a regular fluorescent works fine, but I do try to let
my jars get some natural sunlight whenever possible -- making sure, of
course, that the jars don't get too warm.]


CROPPING:
Relative Humidity 85-92%
Air Temperature 74-78 F.
CO 2 less than 5000 ppm.
Fresh Air Exchanges 1-3 per hour.
[but be careful not to contaminate
'em!]
Flushing Pattern Every 5-8 days.
[this means a new crop or "flush" of
shrooms should appear every 5 -8 days.]
Harvest Stage When the cap becomes convex and soon after the partial veil
ruptures.
Light Indirect natural or same as above.
[hint: use same
as above.]


Moisture Content of
Mushrooms:
92% water
8% dry matter
P. cubensis have up
to 1% psilocin and/or psilocybin per dried gram.
[I would estimate
approx. double that for fresh
'shrooms]





Notes

A quote I recently saw from the Oss & Oeric book reported that a 10 - 12
milligram dose of psilocybin is contained in about .5 grams of dried shrooms (
approx. 50 grams fresh weight). However, 10-12 mg is a HEAVY dose, and it's
ALWAYS best to start with smaller doses -- you can always take more the next
time you trip.
I would recommend then, that you cut this dose in half. The Oss & Oeric
book reports that 2 - 3 dried mushrooms contains approx. 4 mg of psilocybin. For
fresh 'shrooms, I think a good dose to start with would be 3 medium-sized
shrooms. What's "medium-sized"? Well, I don't know, but let's say it's one with
a stem that's about 3 inches long and almost as thick as a drinking straw, with
a cap that's about the same diameter as a penny. Remember when experimenting
with dosages, esp. if you haven't tried fresh ones before, that it usually takes
at least 15 minutes before you notice any effects at all.
If the effects don't seem to be very strong, even after 30 minutes or an
hour, I would still advise against taking more. I think one of the dumbest
things trippers do is to try and strengthen their current trip by taking more.
That's just asking for trouble in the form of an overwhelming/bad trip. Besides,
your judgment probably isn't that great in that buzzed state.
I wish good journeys to you all.





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A valuable and fun source of information on the cultivation of the psilocybe
cubensis mushroom. The step by step 1,2,3 format makes it a useful enough
reference even for the lay person not completely familiar with the methods of
sterilization and aseptic technique which is the main obstruction to cultivation
of mushrooms in general.
Psilocybin:
Magic Mushroom Grower's Guide Home Page





Psilocybin Mushrooms of the World:
An Identification Guide

From the author of Growing Gourmet and Medicinal Mushrooms comes an
identification guide exclusively devoted to the world's psilocybin-containing
mushrooms (the ones that produce a high).
Detailed descriptions and color photos for over 100 species are provided.
With an exploration of their long-standing use by ancients and their continued
significant to modern-day culture.
Psilocybin
Mushrooms of the World Home Page






The Mushroom Cultivator:
A Practical Guide to Growing Mushrooms at
Home

This book excellently describes exact details for mushroom cultivation in a
very understandable fashion. It gives precise parameters for several commonly
cultivated mushrooms and a lengthy list of common contaminants and their cure.
The book gives methods for small time cultivation as well as professional
large-scale production. In general, it is a great primer on the subject of
mushroom cultivation.
The
Mushroom Cultivator Home Page





The Psilocybin Production:
Producing Organic Psilocybin in a Small
Room

Includes closet production and explains small and large scale production. How
to locate magic mushrooms, develop stock for inoculation, cultivate, harvest,
and dry 'shrooms.
Explains how mycelium can be grown in inexpensive jars and methods of
extracting and using existing cultures to seed new one to create an ongoing farm
yielding a regular crop of hallucinogenic mycelium.
The
Psilocybin Production Home Page

12 Aralık 2011 Pazartesi

Oldtimer's Straw Tek


A method I use might prove useful to you. I simply start 1/2 pint jars using traditional PF Tek. Once jars are fully colonized I get ready for straw inoculation. I soak regular old straw (which I get from a Feed & Seed) for 24 hours. After soaking I chop the straw and place in a large pasta pot. Bring to 160F for 1 hour. After pasteurization I allow straw to cool, drain and place in pans with clear "greenhouse" type lids. Take the "cake" and grind it to powder in a food processor/blender. Believe it or not the grinding does not harm the mycelium. Sprinkle the cake powder all over the straw and mix in throughly. Every minute particle of PF cake dust will become a growth point giving super fast colonization of the straw. Allow spawn to run through and case. You will get really good flushes (much more than fruiting individual cakes) and produce many more carpophores for your enjoyment. Of course optimum humidity must be maintained throughout the entire growing cycle from inoculation to fruiting. For years I started cultures using petri dishes, replated several times, transferred to grain and fruited strictly on manure/straw compost. Now, thanks to PF I have learned a much easier and quicker method for growing our fungi friends using straw and cakes with no lab time at all. See, you can teach an old head new tricks.

summary by Kaijan

Materials:

· Straw
· Miracle Grow / Potting soil (peat based mix)
· Lime (Mineral)
· Pans with clear lids.


Procedure:

1. Soak straw in water for 24 hours
2. Chop the straw and place in pasta pot, boil for an hour at 160F
3. Drain Excess water in colander and allow it to cool off.
4. Place straw in pans with clear lids.
5. Take a ½ pint PF style cake and crumble or grind up into small particles.
6. Sprinkle powder over the straw and mix thoroughly.
7. Allow spawn to run through and case.
8. Maintain optimum humidity through growing cycle.

For Casing:

7. Obtain good potting soil (miracle grow, or a peat based mix.)
8. Add a small amount of lime (mineral) to raise Ph
9. Place soil mix in a pan and heat in an oven to 160F for 1 hour.
10. Allow soil to cool off completely.
11. Mix soil with water until damp. (No water drips when squeezed)
12. Spread a layer of moist soil ¾�s of an inch deep over the straw.
13. Mist 2-3 times a day to keep soil moist
14. Keep humidity high, but do not over water.
15. Fruiting should occur within a week.

Quote:

more...
========================================
I use a good potting soil such as "Miracle Grow" or any peat based mix. Take dry soil and mix in a little lime (mineral, not fruit!) to raise the Ph slightly. A little will do the trick. Place the soil mix in a pan and heat it in an oven to 160F for about 1-2 hours. Allow soil to cool and mix with some water (preferably sterile) until you get an overall moistening. You want it to be damp, but not soaking wet. A good rule of thumb is to wet until it forms a good ball when you squeeze it in your hand but no water drips out. Just good and moist. Spread a layer of moistened soil about 3/4 inch thick over the spawn-run straw, keep moist and mist the casing lightly with water 2-3 times a day. You should begin to see pinning within several days. Keep the humidity high but do not overwater. You want it moist, not dripping wet. Think like a mushroom... Ahhh, this feels just right... and you'll get a good crop. I can turn you on to a really fast manure/straw compost method that produces compost in seven days if you want to try it, but the straw alone will work fine.

yet more...
==============================================

Geez Quote.... Where do I start?!

Q: Is it more effective to pasteurize wet rather than dry materials?
A: Hell if I know. I do know it's a lot easier to stir a pan of 160 degree dry soil in the oven. In short, the heat is doing its job wet or dry and it's much easier to work with dry. An additional plus is the fact that you can store the dry stuff for later use. Wet soil is much less convenient to store and is more likely to be infested with bacteria if you tried to use it later. I like being able to sterilize a batch of dry soil, use what I need and put the rest in a clean zip-loc freezer bag for later use.

Q: Why do I prefer a peat based mixture that requires the Ph to be adjusted?
A: Well to be honest... I've always used peat based soils for casing because that's what Bob Harris and Paul Staments taught me to use about 25 years ago! Actually though, I really like the "consistency" of these soils. They hold moisture nicely, stay fluffy and are easy to spread over the spawn. I assume vermiculite would work but may be hard to wet evenly if spread on dry. You could probably wet it in a bowl (like you do when fixing up PF jars) and it would do okay. It's just a matter of what works for me as well as ease of use. As for Ph, well it's no problem to throw in a pinch of lime. It's not like you have to get a Ph pen and really adjust the medium to an exact Ph. The lime just pulls the Ph up a little and takes the acidic edge off. I have used peat soils with no lime, but I do seem to get faster/better pinning with a little lime.

Q: If one did use a 3/4th inch thick casing, what depth of substrate would you recommend?
A: Depends on how deep your container is! Seriously, I usually place about 3-4 inches of straw in the pan and case with 1/2 to 3/4 inches of soil. You don't want your straw too deep since you're wanting the spawn to colonize rapidly. A really deep container of straw tends to compact reducing spawn run plus it can get so dense it can actually mat allowing anaerobic bacteria to crop up ruining your bed. You don't need a thick layer of straw to grow an abundance of shrooms.

Q: Have you fruited directly from straw, without casing?
A: Yes.

Q: I'm told that straw doesn't need to be cased, why do you prefer to case it?
A: Open straw will pin and fruit, as will PF cakes, compost or whatever else you find to grow on. The reason for casing is easier moisture control. Pure and simple. The casing layer acts as a buffer between the air and the spawn effectively creating a micro-environment just under the casing. This protects primordia that are forming and prevents aborts due to excessive drying. Plus you can mist the casing layer without applying water directly to your spawn. The shrooms will thank you for this. By using a casing layer you can eliminate a lot of the stress involved in trying to keep bare cakes or whatever from drying. The casing makes your mushrooms a little more "forgiving", plus you don't need as wet an environment thereby helping to reduce the growth of unwanted fungi and bacteria in your beds.

Now for Infoseeker:
Q: How many PF cakes to use?
A: I normally grind up and spread one 1/2 pint jar per square foot of bed. My beds are generally about 3-4 inches deep but one jar should handle beds of 6 inches in depth. Be sure not to get to deep are you'll have unwanted guests from the anaerobic bacteria community. By the way, while we're discussing bacteria, please be really careful handling spawn or anything else that has become contaminated. Some of the critters you might find growing in your jars can make you quite sick if you happen to breath them in or inadvertently ingest them. Dispose of any and all contaminated cultures immediately.

As for the hydrogen peroxide process for bulk spawn preparation I have to refer you to the resident expert on the subject, R. Rush Wayne, Ph.D. His booklets titled "Growing Mushrooms the Easy Way" "Cultivation with Hydrogen Peroxide" Volume I and II can be purchased from him at mycomasters.com. I have read both volumes and find that problems related to peroxide-decomposing enzymes in organic substrates can limit your choices. He spells out ways to work around this but I have found hot water pasteurization to be an easier route. If you can swing it set up a clean 55 gallon drum on concrete blocks and place a gas "fish cooker" under it. Make a basket out of metal hardware cloth and heat bulk quantities of straw this way. I have never needed that much straw for psilocybes but I do pasteurize straw this way for Pleutotus (oyster mushrooms), Portobellos and other gourmet shrooms that I grow.

Q: Does anybody sell ready to use compost?
A: Check with MushroomPeople and Fungi Perfecti but don't mention psychedelics as they probably won't deal with you if you do. Personally, I would suggest making your own compost if you have access to cow manure and straw. It's very simple and I know a super quick trick to get usable compost in 7 days. I'll post the method when the "writers cramp" moves out of my fingers! Later.


and...
==============================================


Infoseeker,

If you are placing perlite under your straw it probably IS more trouble than it's worth. A good, properly prepared straw bed 3-4 inches deep shouldn't need any additional humidity or moisture retaining additives. The straw itself is moist and should hold more than enough water to produce plentiful mushrooms. My beds stay moist (especially after casing} and the mycelium grows fast and furious. You should strive for a light and airy bed with ample (but not stifling) moisture content. Beds that I inoculate with ground up PF cakes fully colonize in 2 days to the point where you can literally lift the entire bed out as a block or brick of mycelium. Inspection under the straw reveals a moist, healthy environment with no contaminants at all. I think folks are getting too involved with environment control and missing the point (to produce carpophores). Mushroom farming is like any any other growing project. Sometimes folks get so wrapped up in creating environments they overlook the simple, common sense things. Trust me, pasteurize some straw, drain it and place in a shallow pan with some type of lid. Inoculate this straw with a jar or two of ground up PF cake powder as spawn and watch what happens. Let go of all the processes and procedures you've read about and let nature take its course. Please don't think I'm trying to sound like some mushroom guru or something, I'm not, I'm just relaying knowledge to you that has accumulated around me over the years. Simple methods work and simple is always better if you achieve the desired results.

If you plant a bed in the method described above you should have no need for hydrogen peroxide or any other sterilants.

I will post hydrogen peroxide substrate preparation steps as explained by R. Rush Wayne, Ph.D. for straw since you seem to be interested in trying this method. I still say hot water pasteurization is easier and less of a hassle, but this forum is for the dissemination of information and on that note...

The following excerpt is from...
GROWING MUSHROOMS THE EASY WAY - Home Mushroom Cultivation with Hydrogen Peroxide
VOLUME I by R. Rush Wayne, Ph.D. Copyright 2000
Complete copies are available at www.mycomasters.com

In Volume I he suggests using Calcium Hydroxide to pasteurize straw.

Growing Mushrooms on Straw Using Hydrated Lime --

A number of mushrooms will grow on straw, and straw is the traditional substrate for oyster mushroom cultivation. The standard way to prepare straw for mushroom growing is to steep it in water heated to 180 degrees F for an hour, then drain cool it down and inoculate. However, without some specialized equipment, it can be rather awkward to heat any significant amount of straw in hot water, and it is often difficult to tell whether the straw has cooled sufficiently for inoculation unless you have a compost thermometer. So, I prefer to use a cold water hydrated lime soak. Hydrated lime (calcium hydroxide or Mason's lime, available at builder's supply stores) is a caustic powder which creates a strongly alkaline solution in water. The alkali both pasteurizes the straw and wets it by penetrating the waxy coating on the straw. When the solution is drained off, the liquid that remains behind on the straw gets converted by carbon dioxide in the air to ordinary calcium carbonate lime, which is alkaline but no longer caustic.

I have a large plastic tub that will take a quarter of a bale of straw. With the straw in place, the tub then takes 25 gallons of water to fill to the rim. I use a one half percent solution of hydrated lime, which means one pound of lime in 25 gallons of water. I mix a slurry of hydrated lime in a separate bucket, then pour it into the tub in stages as the tub is filling with water, to assure thorough mixing. (Caution: hydrated lime will burn skin and eyes. Be sure to wear gloves and eye protection when handling the powder or the solution. Also store the powder in an airtight container to maintain its potency). I let the straw soak for about 16 hours, then drain it for 2 hours. You can probably get away with using half as much lime, but then it may prove wise to soak the straw somewhat longer, say 24 hours instead of 16.

I inoculate the straw by filling a plastic trash bag, draped inside a large cardboard apple box. I put a layer of straw, then a sprinkling of spawn, then another layer of straw, then more spawn, and so forth. Finally I close up the bag and compress the straw by pushing the bag and its contents down into the box as far as I can get it to go. A quarter of a bale of straw fills three apple boxes worth. The bags get loosely twisted closed and covered with old bed sheets to incubate for about a month.

In Volume II he suggests using Hydrogen Peroxide to pasteurize straw.

Bulk Substrate - Preparing straw with peroxide at room temperature--

Next we come to a method for using peroxide to prepare straw for use as a mushroom substrate. This method can be carried out entirely at room temperature, with no heating and cooling step, and no caustic solution required.

Here I have to admit that my previous publications all argued that straw could not be usefully pasteurized by treatment with hydrogen peroxide solution. I reasoned that straw contains high levels of peroxide-decomposing enzymes (as do other similar substrates), and these enzymes would both destroy the peroxide in short order and protect the numerous mold spores in the straw from the peroxide. Now it turns out that straw nevertheless CAN be pasteurized with hydrogen peroxide. Yes, the peroxide is indeed destroyed by the enzymes in the straw in a relatively short time. But if we raise the peroxide concentration and we tweak the chemistry of the peroxide solution slightly, the peroxide can still have a beneficial effect even in the brief time it survives contact with the straw. And although the peroxide itself does not linger to protect the straw from subsequent contamination, it nevertheless seems to transform the straw into a substrate that is favorable for the growth of mushroom mycelium, one that resists contamination even when the peroxide itself is gone. The peroxide apparently does this at least partly by way of a chemical reaction with the straw.

Despite this complicated explanation, the protocol for preparing straw with peroxide is extremely simple. It goes like this:

1. Place your straw in a large soak vessel.

2. Fill the vessel with a hydrogen peroxide concentration of at least 0.15%, combined with 10 mls. of vinegar per liter of soaking solution (about 2.5 tablespoons per gallon).

3. For chopped straw, soak about 4 hours at room temperature. For unchopped straw, soak for at least 28 hours, or until the leachate takes on the color of a good tea.

4. Drain the straw throughly, until it is no longer drippy.

5. Remove the straw to your culture containers, mixing in spawn as you go.

I recommend chopping the straw for best results. Chopping the straw promotes more efficient absorption of water, and the smaller particle size encourages faster mycelial growth upon inoculation.

- - - - - - - - - - - - - - - - - - - - - - - - -

By Fungusflipper (Fungusflipper) on Saturday, May 05, 2001 - 06:14 pm

So, guys, my FOAF wants to try the straw tek Old T talks about, but I am curious.... being a first timer should he use the plastic layer over the spawn while it germinates? I know another straw tek mentioned this.... Do you guys feel this step is important or no?

FF

By Quote (Quote) on Saturday, May 05, 2001 - 11:46 pm

i would, it keeps moisture right.

By Oldtimer (Oldtimer) on Sunday, May 06, 2001 - 03:18 pm

Yeah, you need something to keep the humidity in. I use growing trays with a clear top that creates a kind of "greenhouse" environment inside the tray.

Let us know how it goes...

OT

By Fishy1 (Fishy1) on Sunday, May 06, 2001 - 03:42 pm

Hey OT,

Are you referring to the black plastic garden flats that have the clear plastic top? These are avail. at local "mart" stores...

And what is the scoop on perlite. Yrs ago I visited a shroom farm in the SW. The growers had 3 huge greenhouses going. Birdseed, onto straw, cased with peat, vermic, and perlite. The caps were like hamburger buns! People on this site seem to have an aversion to perlite, it seems...what gives? fishy1

By Oldtimer (Oldtimer) on Sunday, May 06, 2001 - 05:10 pm

Those are the ones, I use 'em all the time for starting straw beds... Perlite bugs me due to its habit of being hard to get rid of... trashes up the place and is really noticable in beds. Makes a nice additive for casing mixes in limited amounts.

By Quote (Quote) on Monday, May 07, 2001 - 02:03 pm

we luv perlite, really

-------------------------------------------

I hope this answers some of your questions - Nan

Shroom Glossary

6 Aralık 2011 Salı

Simple Bulk Spawn Production Tek


PSiLO187's Simple Bulk Spawn Production Tek


Ok before you say "Oh my God, another stolen idea tek" don't... I am fully cognizant that everyone is aware that there are many bulk growing styles and that they vary as well. The (sole) purpose of this TEK is to help newbies that wish to take this hobby a step further as far as production and beauty is concerned. The way this tek is intended to help by overcoming the fear of crossing the bridge between "familiarizing" themselves with growing principles via the PF-Tek and all its affiliates (Neglect Tek, Sea-Monkey Tek, or even the "Dunking for Cakes" Tek). We all agree that the community would definitely not be where it is today without these valuable contributions to helping others learn the magic art of mushroom cultivation. The only thing that I did was actually took the time to write this. After years of growing I have found this to do mushroom cultivating justice. Enjoy.

Jar Preparation and Assembly:

Ok assuming you have already learned how to cultivate via the PF Tek, lets talk a little bit about jars. The ½ pint will no longer do for production if you are planning on using spawn. The best jars I have found are the ½ gallon wide mouth jars that you can purchase from many different sites on the internet or go to a local hardware store (Ace Hardware has been known to have them). You will be able to fit 4 ½ gallon jars in most 22quart and larger p/cookers, that is 2 gallons of grain per cook (think about it). Another thing you will be using is either Poly-Fill (synthetic cottony stuffing for pillows) which is available at almost any Wal-Mart or wide mouth filter disks that are sold from Fungi Perfecti (www.fungi.com) or The Mushroom People (www.mushroompeople.com). Many people prefer to buy the plastic lids available at fungi.com versus the metal lids, either will be fine though. The first step in creating a new shake jar is to drill a hole in the mason jar lid. Make the hole about the size of the end of your pinky finger (maybe about .4 of an inch). Next you will pull out a hand-full of Poly-Fill and pull it TIGHTLY through the hole. It is a good idea to pull of too much excess Poly-Fill, the only reason it is there is to keep out contams, not fill the whole jar. If you are using filter disks simply make the hole a little larger and put the disk under the mason lid.

Grain Preparation (to be used as spawn)

Now that your jar lids are fully assembled you are now ready to see the beauty of grain spawn. First and foremost you must realize that various grains usually work fine so you will be able to mix and experiment with this as you start growing in this fashion, but to make this tek simple lets just say we are using Rye or Barley. Ok some people prefer boiling (or steeping rather) their grain, some prefer to soak for a day or more, and some even prefer to just add a given amount of water straight into the jar and pressure cook with the grain. The reason this is done is to hydrate the grain and release endospores that are known to cause contam problems. All of these methods seem to work, but I prefer to use the soak 24-48 way. I do this because it is so simple and requires no thought. Basically you want the grains to have water in them and not have them explode or burst or get too soggy. Using the pressure cooker with no lid or even a bucket does this fine. Whether you put the grains in hot water (not quite boiling) or soak overnight the main thing you will have to watch out for is starch. After I have prepared the grain bye steeping or soaking I ALWAYS THOROUGHLY RINSE all the slimy carbohydrates/starch and funk off of your grains and DRAIN *VERY* THOROUGHLY before you pressure sterilize your grains. The reason for this is because you will have a fatty caramelized mess in the bottom of your jars when they cool, which prevents the nifty shaking feature of grains jars and slows rhizomorphic Mycelial networking.

Filling the Jars

This is pretty simple. Fill the jars between 3/5 and 3/4 full. The grains will expand as they are sterilized somewhat, depending on how water logged they are. Screw the lids on of course. Put foil over the lids the same way you would in the PF TEK to keep water out during the pressure sterilization. I recommend using masking tape to hold the foil on, but this may not be necessary, I do it out of habit though.

Sterilizing the Grain

After you have filled the jars you are ready to sterilize the grain. I am sure you have heard about many different times being uses to sterilize grain. I have only used one cook time as far as I can remember and I have had success with it so I had no reason to try any other. 70 minutes at 15psi, but when I say 70 minutes I am speaking of AFTER I hear the first small hiss coming out of the pressure cooker. After the 70 minutes is up I take the cooker off the burner and let it cool down (as much as possible) and then take the lid is off the pressure cooker. I prefer to take the jars out of the pressure cooker warm or even HOT to make sure the grains are not sticky and are shakable before the jar cools down. The grains tend to break up easier if the jars are still warm. Let jars cool 100% before going to the next step of course...

Creating Liquid Inoculant

I have read that allot of people use honey and sugar water to grow mycelium for inoculations. I find this to be more time consuming than I prefer. I actually prefer to clone strains I like from mushroom tissue, but as this is a simple tek I am leaving all that out (you can do research if interested in cloning with agar, that is a whole other project all together.) When I had no petri dishes or cloned mushroom tissue all I did was make water out of pre-existing colonized substrate. The easiest way to do this is to is to put some pebbles, coins, and/or small objects of any sort that are able to sink to the bottom and tear the mycelium once shaken aggressively) into a 1 quart jar full of spring water and sterilize. I poke 1 hole in the side of the lid to draw the water out into the syringes and cover lid with foil as usual. Wrap 1 spoon, 1 fork, and 1knife (100% metal, no wood) in foil. Most canners come with another rack that can go on top of jars; this is where the wrapped fork n knife will go, out of direct contact with the water. These sterilized eating utensils are what you will be using to scrape mycelium and drop it into the cool sterilized water. Once everything is cooled down 100% select a good jar of colonized grain, PF-TEK BRF cake, or even birdseed and make sure it is not contamed at all. Turn off your AC/ Heater in your house and spray Lysol EVERYWHERE around where you will be working. And wipe all surfaces as usual. Take the lid of and with the sterilized tool of your preference scrape the layer of vermiculite off if necessary, as it is trash and not colonized. Scoop a healthy few scoops of mycelium colonized substrate into the sterilized water (I use the spoon), and re-attach the lid. The foil I originally covered the lid with in the pcooker I put back on the jar. I place my finger over where the hole is (the foil separates my finger from the water) and I shake like a sick puppy. Never failed me once! The thing to watch out for, though, is using the same strain over and over, generation after generation, and not doing it from a master; this causes the strain to degrade after 3 or so times.

Inoculating with Liquid Inoculant

This is the easiest part. In a very clean environment draw water out of the quart jar containing the liquid inoculant. I pretty much use 1 whole syringe per ½ gallon jar. But make sure that you don?t add TOO much water, or your risks of bacterial contams are higher. If using Poly-Fill insert the needle between the side of the lid in the hole and the actual Poly-Fill (Don?t insert it smack dab in the middle of all the polyfill material). If using filter disks crack the lid open barely (don't breath in there!) and squirt, squirt, squirt. Viola!

Inoculating with Grain/Substrate Transfer

Simply take sterile colonized substance and transfer it into the virgin spawn. Must be very sterile conditions, use the tools you sterilized. There are teks out on this in detail as well.

Colonizing and Shaking of the Jars

The more you shake the jars is not necessarily the better they will do. Shake the jar once every 2 days or so. Once the grain has mycelium pretty evenly distributed it is time to stop touching the jars all together. They should be colonized within 2 weeks (give or take a little).

Creation of Bulk Substrate with Straw and Worm Casting Compost

Okay, this is where all the fun really begins. I have experimented with different types of growing medium and found both wheat straw and worm casting compost to be the best. Both do fine by themselves but worm castings seems to do better than straw. But the straw is so incredibly cheap per bail and the casting compost from mushroompeople.com costs a bit more because of shipping. So to this my recommendation would be to mix the 2 together. It is very simple.

Pasteurizing the Straw

First cut the straw as small as you can get it 1?-2? is fine; Shears, scissors, whatever. Get an old pillow case and put straw in it and tie a string around the top of the pillow case to hold it all in. Put this pillowcase in the base of a large pressure cooker or some other large metal container that can withstand the stove-top. Fill this with water and set a weight on top of it to prevent it from floating. You will need to keep the temperature close to (***but definitely less than***) 200f. If you go over this you are destined to failure. Let it cook in this for 2 hours and make sure there is always a good amount of water in it so it will not burn. A candy thermometer is a good way to tell what temperature it is in the pot, you can buy these at Wal-Mart. After it has been 2 hours take this out and let it cool off *100%* and make sure that is THOROUGHLY DRAINED.

Pasteurizing the Worm Dung

This is pretty simple. The hardest part is getting the water ratio right. Basically use good judgment and make sure you don?t make muddy sludge just wet it good but not to where it is dripping and shit. Some recommend putting lime in your worm compost, but it is not a very big deal. Put this wet worm shit into large pie tins, put foil over the tins, and preheat the oven to 175f. Leave the tins in the oven around about 2 hours. Let cool off completely, 100%.

Mixing Spawn into the Substrate

Ok this is another cool part. Take a sterile Rubbermaid clothing bin (again from Wal-Mart) and duct-tape the bottom 6 inches of it to block out light from hitting the substrate from the sides. I recommend 1 full pillowcase to a 10 pound bag of compost (they sell them by the 10lb bag at mushroompeople.com) Mix both of these pasteurized substrates into the Rubbermaid Sterilite container. Next Get 2 (or 3 even) 1/2 gallon-sized jars (a total of 1 gallon of spawn) and mix the substrate from the jars into the dung/straw mixture. Make sure you compress the straw/dung mixture as much as possible, if it is TOO fluffed out it is not a good thing. Another adaptation is to take an additional third ½ gallon spawn jar and mix it into 100% worm dung/compost and make a pseudo-casing out of the mixture, this keeps the straw from sticking through the casing layer. Let ALL of this colonize 100%, it wont take long AT ALL. After it is colonized 100% case normally with 50/50 or even coco-fiber. Put a light on the sterilite lid and soup the tank up however floats your boat. Growing parameters are the same of course. Expect a VERY, VERY high yield.
http://www.shroomery.org/9029/PSiLO187s-Simple-Bulk-Spawn-Production-Tek

Growing Bulk: Part 1: Birdseed

Growing Bulk: Part 1: Birdseed


This is the first part of a two part grow guide teaching you how to grow mushrooms in bulk (on straw).

Step 1

First of all, you’ll have to do some shopping. Go to your local supermarket and buy the following (if you haven’t got it yet):
  1. Birdseed without sunflower seeds or corn
  2. Tin foil
  3. A cooking pot
  4. A couple of pint glass jars with metal lids
  5. Micropore tape
  6. Rubbing alcohol
  7. Latex gloves
  8. Mouth mask
Also, buy a spore syringe online. Click here for a list of suppliers of edible mushrooms.

Step 2

Take your birdseed and soak it in hot water for 24 hours. Take out any floating seeds. Yes, every single floating seed has to be taken out. These often are hollow seeds and can contain contaminants. Next, put the birdseeds in a colander and let them drain until the seed shells look dry.
The seeds should still contain their moisture, but when put on a paper towel they can’t leave any wet spots.

Step 3

Now it’s time to take out your glass jars. Unscrew the lids and puncture 4 small holes in them using a nail and a hammer. The holes should be about half an inch from the side.
Grind down any metal that’s sticking out. You can seriously hurt your fingers with that.

Step 4

Fill the jars with the drained birdseeds. Leave about two inches of space from the top of the jar. Put the lids on. Stick micropore tape over the holes.  Wrap your lids and jars with tin foil. Make sure you don’t make any holes or tears in it, that is crucial.

Step 5

Put your jars in the cooking pot. Fill the cooking pot with water until the jars are halfway under. Put the water to a boil for about 2 hours with the lid on the pot. Make sure there’s always some water present in the cooking pot.
After this, take the jars out and let them cool down to room temperature. The simplest method is to leave them to cool over night. Remember that the inside of the jar is hotter than the outside. So it’s better to wait a bit longer. 12 hours should be sufficient. Leave the tinfoil on the jars!

Step 6


Take the rubbing alcohol and wipe your entire work area clean. Your work area should be under a flow hood. If this is impossible, work in a chamber where there’s no moving air or drag.
Take your jars into your work area, take the tinfoil off and rub the outside down with rubbing alcohol. Put on your latex gloves and mouth mask and rub your gloves with the alcohol. Now your completely sterile and ready to work.
Take the spore syringe you ordered online and hold the needle into a flame until it’s red hot. Then wipe it with some rubbing alcohol to completely sterilize and cool down. Now gently stick the needle inside one of the holes in the lid, through the micropore tape in one fluid motion. Tilt the syringe so that the needle touches the side of the jar. Gently insert 1cc of spore solution into the jar. Repeat this process for all holes of all jars.
Always take your needle through a flame and clean it with some rubbing alcohol in between two jars.

Step 7

Now place your jars somewhere where it’s not too bright. The jars should stay at a constant temperature. For most fungi room temperature should be fine. The jars should stay there for about 21 days. Until you see that the birdseed is fully colonized by the fungus.

Sadly, growing mushrooms on birdseed isn’t profitable, so we’re just using these colonized birdseeds to spawn (=fungi mycelium used to inoculate other substrates)  some straw in part 2 of this bulk grow guide.


Growing Bulk: Part 2: Straw

Growing Bulk: Part 2: Straw


This is the second part of a two part guide on how to grow your own mushrooms in bulk. You already have your halfway colonized jars from part 1. Now we’re going to grow on straw. In this part we’re actually growing the mushroom.

Step 1

I presume that you still have the materials from part 1 of this guide so this is what you need to buy for part 2:
  1. Straw
  2. 2 large containers to put your straw in (a rubbermaid for example)
  3. Dish soap
  4. Hydrated lime
  5. A thermometer (up to 212°F)
  6. Large plastic bags

Step 2


Cut your straw into small pieces between 1 and 3 inches and put it in the large container. Add boiling water until the straw is fully submerged. Add some dish soap. Mix it up real good and let it sit for 2 hours.

Step 3

Again boil some water and put it in the second container. Add 1 cup of hydrated lime for each 10 gallons of water. Now transfer the straw from the one container to the other.
Stick the thermometer in. Use some flat object  to submerge the straw completely (a plastic lid from a box will do fine). Temperature should stay between 90°F and 140°F for 1 hour.

Step 4

Take your jars and shake all the colonized grains lose. Hitting the jar against a bike tire or a roll of sticky tape helps, but be careful not to smash it.

Now take the plastic bags and open them up. Grab a hand of the straw, shake the excess water off and put it in the bag.
Then put some of the colonized birdseeds on top of the straw. Then again a layer of straw, and again a layer of seeds. Repeat this until your bag is filled. Make sure that the bag is filled tightly so press the straw together until it is packed together nicely. Make a knot in the bag so that it is tied shut. Now wipe the entire bag with rubbing alcohol.
Then you’re going to make a few holes in the bag for air exchange. Make sure the knife you’re using is rubbed with alcohol. The holes should be about 5 to 6 inches apart.

Step 5

Place the bag horizontally for about 2 weeks at room temperature until the fungus has fully colonized the straw.

Step 6

Once your bag is fully colonized it’s time to build your fruiting chamber.
Take one of the large containers and drill 1/4″ holes in it on all 6 sides, 6 or 7 inches apart. Then gently cut away the plastic bag with scissors careful not to damage the fungus mycelium. Place the straw log inside the container.
Place the container in a bright spot at room temperature. Mist some water inside the container to maintain high humidity levels. Be careful not to overdo your watering. You just need some condensation on the sides of your container.

Step 7

In about 2 weeks you should be ready to pick the fruits.
Below is an image of Psilocybe cubensis growing on straw.




3 Aralık 2011 Cumartesi

THE PSILOCYBIN PRODUCERS GUIDE


THE PSILOCYBIN PRODUCERS GUIDE

How to produce 5000 doses of organic psilocybin in a small room every week

by
Adam Gottlieb
1976

INTRODUCTION

If a person knows what he is doing, It's not difficult to cultivate the mycelium of any of the psychoactive psilocybin bearing mushrooms. The mycelium is the fibrous underground network of the mushroom. The familiar stem and cap portions of the mushrooms are called carpophores. The mycelium can be readily grown in ordinary Mason jars in a low cost medium in 10 to 12 days and the active materials (psilocybin and psilocin) can be easily extracted. This Guide shows how to carry out all of these steps on a small or a large scale. Complete instruc- tions are given for locating the mushrooms, developing stock cultures for inoculation, cultivating, harvesting, and drying the mycelium, extracting the active alkaloids, and using the existing cultures to seed new cultures to keep an ongoing psilocybin farm yielding a regular crop of the hallucinogenic mycelium. We also give directions for setting up in a small workroom a large scale psilocybin factory which can produce at least 5,000 doses of the drug every week.

PSILOCYBIN AND THE LAW

The present drug laws are a pathetic mess. The old adage that ignorance of the law is no excuse becomes a ludicrous statement when the laws themselves are rooted in ignorance. One classical example of this is the classification of the stimulant Cocaine as a narcotic. One is reminded of the King in Alice in Wonderland who made up his own language as he went along with total disregard for the accepted definitions of words. I will not even go into the question of whether any law enforcement agency has the moral or Constitutional right to dictate what substances we may or may not take into our own adult bodies. Any modern individual whose mind is not immersed in the slavish dung pit of Dark Age unreasoning knows that reliable education - not criminal penalization - is the answer to whatever drug problems exist. Nevertheless, we must contend realistically with the powers that unfortunately be at this time. They are the ones with the badges, guns, gavels and goons.
Because of the afore mentioned ignorance of our lawmakers it is difficult to determine how the use of certain hallucinogenic substances would be treated in the courts. Possession of psilocybin and psilocin (misspelled in the U.S. Code as psilocyn) is a felony under Title 21, Section I, (C) of the United States Code (1970 Edition). Psilocybe Mexicana is also illegal. There was sufficient ignorance on the part of the lawmakers not to include the many other mushroom species containing psilocybin and psilocin. Theoretically the possession of any psilocybin bearing mushroom would be the same as possessing the alkoloid itself. But when it comes to prosecution it does not necessarily work like that. Lysergic acid amides, which occur in morning glory seeds, stems, and leaves are also illegal, but there is no way to prevent gardeners from raising this ornamental flower. It is also illegal for anyone in the USA to possess mescaline. Peyote, which contains mescaline, is legal for bonafide members of the Native American Church when used ritualistically, but no member may possess extracts of the cactus or the drug mescaline. Peyote is illegal for non-members, but San Pedro and several other species of Trichocereus cacti also contain mescaline and are available from many legitimate cactus dealers. It would be clearly illegal for anyone to extract the active principles from any of the above mentioned plants. And it would be illegal for anyone to extract psilocybin and psilocin from mushrooms or mycellium as described in this guide. Anyone found operating a large scale mycelium farm could very easily be prosecuted for intent to manufacture psilocybin and psilocin. There are also many different state laws which must be considered before doing anything psilocybin bearing mushrooms. There are, however many Nations which have no laws regarding these substances. We are not judges or attorneys and are not trying to offer clear interpretations of the law. Rather we have mentioned these points to give some indication of the legal pitfalls which surround the application of the activities described in this guide. Furthermore, laws may have changed for better or for worse. We, the author and publisher, are not recommending or endorsing the application of the information in this guide especially in places where there are laws proscribing these substances. We offer this information for the sake of pure knowledge because it is our Constitutional right to do so. We do not encourage the violation of any existing laws.

FINDING THE MUSHROOM

All it takes is one mushroom or a few spores and from this one can quickly develop a culture that will continue to produce as much psilocybin as one desires for years to come. Because the common San Ysidro mushroom, Psilocybe cubensis ..Singer (Formerly Stropharia cubensis ..Earl) is the most easily obtainable, the most readily cultivated, most disease resistant, and psychoactively strongest species we have geared our instructions to it's use. There are, however, numerous other species which contain psilocybin. In case one of these is all that is available, we give for several of these pertinent information such a relative potency, where and when to find specimens, what growing conditions (medium, temperature, lighting, etc.) it favors and how resistant it is to contamination. The states, provinces, and regions named are by no means the only places where the species is to found. They are places in which there have been numerous reports of findings. They are given here to give a general idea of the type of terrain and climate the species favors. In cases where ideal cultivation temperatures and growing conditions are not given much can be surmised by considering the environment in which that species thrives.
Psilocybe cubensis can be found in many parts of the United States, Mexico, Colombia, Australia, and even Southeastern Asia. It is usualy found growing on or near cow dung in pastures during warm rainy periods from February to November. There are several species of mushroom which occur on cow dung, but fortunately none of these bear much resemblance to the San Ysidro.
There are numerous toxic mushrooms growing around us. Some of these could be mistaken for some of the other psilocybin fungi mentioned in this guide. It is essential that the mushroom hunter learn to use an identification key. A key is a listing of the various features which will positively identify a given species. If a specimen does not confirm in every respect to the key, it must not be used. There are several excellent keys to be found on most library shelves. One that we recommend is "Keys to Genera of Higher Fungi" by R. Shaffer, 2nd ed. (1968) Published by the University of Michigan Biological Station at Ann Arbor. We also recommend a thorough reading of the most helpfull book "Poisonous and Hallucinogenic Mushrooms" by Richard and Karen Haard, available for $3.95 from Nature Study Institute, PO Box 2321, Bellingham, Washington 98225. It is further suggested that after identifying the specimen it should be brought to an expert mycologist to be absolutely certain of it's identity.
Many books on hallucinogenic mushrooms suggest a simple test for psilocybian species which involves breaking the flesh of the specimen and waiting about 30 minutes for a blueing reaction to take place. The blueing is due to the oxidization of indole based substances in the fungus. Although it is true that most of the psilocybin-bearing mushrooms will respond positively to this test, other species may do the same. The poisonous Eastwood Boletus blues upon exposure of the inner tissues to oxygen as well as does any psilocybin mushroom. Another test which is often given in mushroom manuals is treating the exposed tissues with Metol, a chemical used in photo developers. It hastens the blueing of psilocybin mushrooms, and supposedly one can do a blueing test with it in a few minutes that would otherwise take 30 minutes or more. Any mushroom, however, which contains indolic substances of any sort will respond positively to this test. Since indole-based amino acids such as tryptophan are found in most living organisms this test is rather useless.
There is actually no field test for psilocybin mushrooms. There is however, a relatively simple test for the presence of psilocin and psilocybin that can be carried out at home by anyone who has some familiarity with paper chromatography. The mushroom sample is dried, pulverized, and extracted into a small amount of unheated methanol by shaking for half an hour. After the debris in the methanol has settled the paper is spotted with the top fluid in a zone about 2mm. After treating the the spotting zone with water saturated butanol for about two hours, the solvent front 7-8 cm from the spotting zone would contain the psilocin and psilocybin if they were present in the specimen. After drying the paper with a hair dryer on warm, this outer zone is sprayed lightly with a saturated solution of p-dimethyl-aminobenzaldehyde in alcohol and then again with 1 N hydrochloric acid. The paper is then dried again as before. Where psilocybin is present a reddish color will develop. The presence of psilocin will be indicated by a blue-violet zone.

DATA ON VARIOUS PSIOCYBIAN SPECIES

CONOCYBE CYANOPES:
Found from May through September usualy in dense shade scattered among mosses, and in wet soil around bogs, swamps, and ditches in the northwestern USA and as far east as Michigan. Carpophores grow well in sphagnum moss having a range of pH 7-8.

COPELANDIA CYANESCENS:
Found in early summer through late autumn scattered, grouped, or clustered on cow dung, or rich soil in Florida and other southern states. Spores germinate easily easily on all agar media. Optimum growth occurs on MEA at 80 degrees F. Carpophores can be produced on uncased compost or on rye.

PANAEOLUS FOENISECII:
(Also known as PANEAOLINA FOENISECII or PSILOCYBE FOENISECII, and commonly known as haymower's mushroom or harvest mushroom) Found in late spring and early summer, or in July, August, and September during cool, wet seasons scattered or grouped in large numbers on lawns, pastures, and other grassy places throughout the USA and in Quebec. Tests on specimens found in Washington revealed no psilocybin, but eastern specimens were potent.

PANAEOLUS SPHINCTRINUS:
Found in summer and autumn in small groups in forests, pastures, fields, and roadsides almost always on cow dung in many temperate parts of the world.

PANAEOLUS SUBALTEATUS:
Found from spring to autumn grouped or clustered often in rings up to two feet in diameter on open ground, freshly manured lawns, straw piles, all types of compost, dung piles, and roadsides in Ontario and throughout the USA (especially in Massachusetts, Maryland, New York, Ohio, Michigan, Washington, and Oregon). Optimum growth on MEA is at 86 degrees F. It occasionally occurs as a weed mushroom in commercial mushroom houses.

PHOLIOTINA CYANOPODA:
Found in August through September solitary to clustered on lawns in such diverse parts of the USA as New York, Washington, and Colorado.

PSILOCYBE BAEOCYSTIS:
Found in autumn and winter, solitary, grouped, or clustered on earth, lawns, mulch, and decomposing forest wood near scattered trees especially conifers - in western Oregon and Washington. It does well on Agar media at 77 degrees F. This is a potent species containing Psilocybin, psilocin, baeocystin, and nor-baeocystin. Perhaps it is because of the latter two alkaloids that it is the most visually hallucinogenic of the psilocybin mushrooms. There is a report that in 1960 a six-year old boy died after eating a large number of these mushrooms. There has never been any other indication that these alkaloids are dangerous. Until there is further clarification of this question, we advise that anyone using this species proceed with caution by starting with small doses and progressing gradually to larger ones. This is especially important when using the extracted crude alkaloids which may contain large concentrations of the baeocystin alkaloids.

PSILOCYBE CAERULESCENS:
Found in the summer during rainy season, grouped or clustered but rarely solitary, mostly in shady places on soil, sugar cane mulch, recently turned earth or stream banks - in Alabama, northern Florida and Mexico. The Mexican variety P. CAERULESCENS var. MAZATECORUM is known locally as "Durrumbe", which means "landslides." There it is often found among landslides, or near corn or coffee plantations. The mycelium does best on MEA at 81 degrees F. Thermal death occurs at 95 degrees F. It is almost impossible to produce carpophores on sterilized rye medium. They can be grown on vegetable compost in dim light, but the incubation period is long (55-85 days). Although this species is resistant to white mold it's long incubation period leaves it prone to other diseases. It is not one of the more potent species.

PSILOCYBE CAERULIPES:
Found in summer and occasionally autumn solitary or clustered on decomposing logs and debris of hardwood trees (especially birch and maple) in New York, New England states, Ohio, Michigan, North Carolina, Tennessee and Ontario.

PSILOCYBE CUBENSIS var. CYANESCENS ..SINGER (formerly STROPHARIA CUBENSIS ..EARL):
Found from February to November in compact groups in clearings outside forest areas, on cow dung, or horse dung, in rich pasture soil, on straw, or on sawdust/dung mixture in Mexico, Cuba, Florida and other southern states. It grows well on MEA at 86 degrees F. Carpophores appear in 4-8 weeks. Thermal death occurs at 104 degrees F. Carpophores larger than wild specimens can be produced by inoculating vegetable compost in clay pots with agar grown mycelium, casing with silica sand/limestone mix, and incubating 4-6 weeks in daylight at 68 degrees F. It does poorly in darkness. It is a potent mushroom and very resistant to contaminants.

PSILOCYBE CYANESCENS:
Found in autumn scattered, grouped, or clustered in woods, on earth, among leaves and twigs, and occasionally on decomposing wood - in northwestern USA.

PSILOCYBE MEXICANA:
Found from May to October isolated or sparsely at altitudes from 4500 to 5500 feet, especially in limestone regions, among mosses and herbs, along roadsides, in humid meadows, in cornfields, and near pine forests in Mexico.

PSILOCYBE PELLICULOSA:
Found September to December scattered, grouped, or clustered on humus and debris, in or near conifier forests in northwestern USA and as far south as Marin County, California. This is a small but potent species.

PSILOCYBE QUEBECENCIS:
Found from summer to late October scattered in shady areas at forest edges, on sandy soil containing vegetable debris regularly inundated by river flooding, and on decomposing wood and debris (especially birch, alder, fir, and spruce) in the Quebec area. It thrives at lower temperatures than other psilocybe species and produces carpophores at air temperatures of 43 to 59 degrees F.

PSILOCYBE SEMILANCEATA:
Found August through September often in large groups on soil, among grasses, in clearings, pastures, meadows, forest edges, open conifier woodlands, and on roadsides - but never on dung - in New York, northern USA, British Columbia, and Europe. Generally regarded as one of the less potent species, but is sometimes quite potent.

PSILOCYBE STRICTIPES:
Found in October rather clustered on soil or on decomposing wood and debris, on conifiers and some other trees in northwestern USA (especially in Oregon). It closely resembles P. Baeocystis, but has a longer stem. It tends to be as visually hallucinogenic as that species and probably contains the same or similar baeocystin alkaloids.

PSILOCYBE SYLVATICA:
Found in September and October in small compact but unlustered groups in woods on leaf mold, debris (especially beech wood), around stumps and logs, but not usually on them - from New York to Michigan and as far north as Quebec and Ontario. This mushroom is small and is often mistaken for P. Pelliculosa.

The species discussed above are only some of the more commonly known ones with hallucinogenic properties. There are recognized among the psilocybin bearing mushrooms 40 species of Conocybe usually ocuring in forests, pastures, gardens, dung areas, sandy soil, ant hills, decayed wood, and charcoal and having a cosmopolitan range; 20 species of Panaeolus found on soil and dung and having a cosmopolitian range; 40 species of Psilocybe found on soil, moss clumps and organic substrata such as dung, rotting wood, bagasse, and peat ranging from the artic to the tropics; and 9 species of Stropharia found on soil, dung and sometimes on leaf mulch and rotting wood and having a fairly cosmopolitian range.

PURE CULTURE TECHNIQUE

The most difficult part of psilocybin mushroom cultivation is the observance of the rules of pure culture technique. These are the sanitary code of mushroom cultivation. There are usually many varieties of bacteria and fungal spores in our environment; floating in the air, clinging to our hands and clothing, issuing from our mouths with every exhalation. Extreme measures must therefore be taken to keep these out of our mycelial cultures, which they would rapidly overrun. The following points should be diligently observed. Work in a clean, uncluttered, dust free room. Immediately before work wash the work table and spray the room with disinfectants. Scrub arms, hands, and nails with disinfectant soap. Wear simple clothing. A freshly cleaned short-sleeve T-shirt is ideal. Gargle with antiseptic mouthwash and cover the mouth and nose with a clean cloth or disposable surgery mask. Cover the hair with a surgical cap or shower cap. Allow no drafts in the room. close all doors and stuff all door jambs. Let no flies, animals, or unnecessary people in the room. Let only sterilized equipment touch the medium or inoculum. Don't lean over your work. Avoid all swift movements that may cause a draft. If possible have a hood constructed around the work table or a screen or curtain surrounding it. Be neat and keep all materials within reach. Keep all equipment about three feet away from the work. Do not permit anyone to enter the room while work is in progress.

STERILIZATION

All utensils used in the cultivation of mycelia must be sterilized by heat before use. Glassware must be boiled in water for 30 minutes. Metalware used repeatedly must be held in a flame until glowing and then allowed a moment to cool before making contact with any cultures or specimens. When the inoculation loop has been used to transfer a fragment of mycelium it must be flame sterilized again before touching the next fragment. All medium containers must be sterilized after the medium has been poured. This process is known as autoclaving. Containers no more than half full with medium are placed in a canning type pressure cooker. The lids of these must be loose enough to allow escape of internal pressures. Otherwise the containers may crack. Seal the lid of the pressure cooker. Keep the stopcock valve open. Using high heat bring the cooker to boiling so that thick steam comes through the vent. Close the stopcock and let the pressure rise to 15-20 pounds. (250 Degrees F.) for 30 minutes. This should be enough to destroy any foreign spores or lifeforms. Any higher temperature or longer period would cause the dextrose or maltose sugars to carmelize. This would inhibit growth and psilocybin production of the mycelium. When the autoclave period is up turn off the heat and let the cooker cool to room temperature. Do not release the stopcock until everything has thoroughly cooled or the sudden change in pressure will cause the containers to boil over. Discard any containers that have cracked during sterilization. Keep all containers of medium at room temperature for three days to see if any foreign molds develop. If they do occur discard the medium in the contanminated jars and thoroughly clean and sterilize such jars before using again.

MAKING A SPORE PRINT

A spore print is a collection of spores on a flat surface. It can serve several purposes. It can be used to assist identification of the specimen by observing its color or if made on a glass slide, by studying the shapes of the spores under a microscope. Mycological identification keys include descriptions of spore prints and microscopic spore features for different species. Spore prints are also the standard method of collecting spores for later germination on agar media. A print from a single mushroom cap contains millions of spores. Many mushroom lovers are now making spore prints on paper from species available in their locales and mailing them to cultivators in other areas where such species are not found. Secret spore exchange correspondence clubs are becoming quite the vogue and will probably be more common in the very near future. A word of caution regarding this practice should be given, however. Do not assume that spores received in this manner are from the species the sender claims they are. If the sender has misidentified the specimen and the recipient cultivates and ingests mycelia or extractions therefrom, the result may be disasterous. Furthemore, I would not put it past some anti-drug fanatic to purposefully disseminate spore prints of dangerous mushrooms to amateur cultivators. This could result in sickness and death for thousands of persons. To make a spore print take a mushroom with it's cap fully opened and gills exposed. With a sharp sterilized blade cut off the stem as close to the gills a possible. Place the cap gills-down on a clean, white sheet of paper, or on a sheet of glass that has just been swabbed with alcohol, or on two or four sterilized microscopic glass slides. Cover the cap with a clean, inverted bowl or bell jar to prevent drying of the cap and intrusion of foreign organisms. Let this stand as such for 24 hours. If a good spore print has not been formed after this time, tap the cap lightly with the flat side of a knife or spatula. This should shake loose many spores. If the print is made on glass, cover it with another glass sheet immediately after removing the cap to prevent contamination. If microscopic slides are used, place two face to face and seal the edges with tape. If paper is used. fold it several times so that the print is well inside.

PREPARATION OF MEDIA

PDA (Potato Dextrose Yeast Agar): Wash 250 grams of unpeeled potatoes and slice them 1/8 inch thick. Wash these several times in cool tap water until the water is clear. Drain the slices in a collander and rinse once with distilled water. Cook the potato slices in distilled water until tender. Strain the cooking liquids through a flannel cloth or several layers of cheesecloth and collect the liquid in a flask. Rinse the boiled potatoes several times with distilled water, add these rinse waters to the liquid in the flask, and discard the potatoes. Add enough distilled water to the flask to make one liter. Bring the liquid to a boil and add 15 grams of agar, 10 grams of dextrose, and 1.5 grams of yeast extract. The agar must be added slowly and carefully to prevent boiling over. While the liquid is hot, pour it into petri dishes or other culture containers. Each should be filled half way. PDY (Potato Dextrose Yeast broth): PDY broth is made in exactly the same manner except the agar is omitted. Mason jars are filled half way with the hot or cool liquid.
MEA (Malt Extract Agar): To one liter of gently boiling water (distilled) add 20 grams of malt extract, 20 grams of agar (slowly, carefully to prevent boiling over), 100 mg of potassium phosphate dibasic (K2HPO4), and 100 mg of calcium carbonate. While still hot pour the liquid into the culture dishes.

EQUIPMENT

Most of the equipment described in this guide is readily available at reasonable prices. One quart size mason jars can be purchased from many stores including Sears for about $2.98 a dozen. If a large scale Psilocybin farm is being set up, a greater number of jars could be obtained from a Wholesale outlet or bought at a discount from the retailer. Pipettes, inoculating loops, petri dishes, agar, and other materials (including pre-mixed media) are found at many scientific supply houses or can be ordered from Difco Laboratories, Inc., Detroit, Michigan 48201. If Petri dishes are not on hand, there are several other containers that can substitute. Baby food jars 1/4 filled with agar media are excellent. Test tubes can be filled 1/3 with hot agar medium, stopped with cotton, autoclaved and allowed to cool while standing at a 17 degree angle. These are known as slants and permit a maximum surface area. A wooden rack can be easily constructed to hold slants at this angle. Baby bottles with a steam sterilizer can be bought almost anywhere. These come in sets of nine or ten bottles. The tip of the rubber nipple should be cut off and a wad of clean cotton pulled through from the inside leaving about 1/2 inch sticking out. The bottles are filled 1/3 with agar medium. After sterilizing the bottles should be kept at a 17 degree angle. A large pressure cooker - the type used for canning and jarring - can be used for autoclaving mason jars of broth medium.

STARTING THE CULTURE

Upon obtaining one or more specimens of a psilocybin bearing mushroom one can begin to cultivate as much of the hallucinogen as is desired. Any part of the fungus can be used for inoculation. If the spores are used, consideration must be given to the natural life cycle of the mushroom. A single cap contains millions of spores, and any one of these will germinate in the medium to form a mycelium. But this mycelium will consist of what is known as monokaryotic tissue. Such a mycelial organism will grow for a while, but unless it mates with another compatible monokaryotic mycelium and forms a dikaryotic structure it will eventually perish. To develop a culture from spores proceed as follows: Scrape the spores from the print into about 10 ml of sterilized water. Shake well. Add 90 ml of sterilized water and shake again. There will be millions of spores in this solution. Have ready several petri dishes or other suitable containers as described previously containing sterilized agar medium. Lift the lid slightly on each container and with a sterilized pipette or syringe place a drop of this spore water on three or four different parts of the agar surface, then cover the container immediately. Let the dish stand at room temperature for 3-5 days. Radial growths of monokaryotic mycelium will occur at each inoculation point. When any two mycelia have grown to the point of making contact with each other mating (somatogamy) has taken place and within a few days these united mycelia will have become dikaryotic organisms. Any portion of this mycelial tissue can now be used to seed new cultures as described later. If a portion of one of the carpaphores gathered in the field is used to inoculate the agar, mating is not required. The tissue of mature fungus is, of course, already dikaryotic. To avoid contamination only inner tissue is used. Place the mushroom cap gills-down on a clean work area at least three feet away from any equipment. Wipe all dirt and slime from the cap with a Q-tip and swab it's whole surface with a seven percent iodine solution. Pin the cap to the Table top by inserting three disecting needles at equilateral points. Sterilize an X-acto blade in a flame, let it cool for a moment, then carve the outer skin of the mushroom. Cut out tiny pieces of the inner mushroom flesh each the size of a match head. Spear each piece with the blade point, raise the lid of the petri dish slightly, press the tissue firmly into the agar surface, and close the lid immediately. Place all dishes so inoculated on the incubation shelf at room temperature. The mycelium must breath as it grows, so do not cap the lids too tightly. When the radial growth of the mycelia appear on the agar surface (3-5 days) these stock cultures are ready for transferring to the broth jars. If any stock cultures are not going to be used immediately, tighten their lids and place them under refrigeration. They can be kept this way for about a year.

RAISING CROP CULTURES OF MYCELIA

The task now is to select the most vigorous appearing mycelia in the dishes. This means the largest and fastest growing specimens and, of course, those not contaminated by foreign molds. Contaminants are not difficult to detect because their appearance differs greatly from that of the mycelia. Mycelia are pure white fibrous mats sometimes having a light bluish tinge. Contaminants may appear as rapid-growing, tiny, white circular spots with blue-green centers, or as surface scums or fuzzy clusters of either gray, black, yellow, green, or blue color. If any contamients appear in any of the culture dishes, discard those cultures. When the dishes containing the choicest mycelia have been selected the mycelia can be transferred from the agar-based stock cultures to the liquid broth cultivation jars. These jars should have been prepared and sterilized three days before transferring and allowed to stand at room temperature during this time to test the effectivness of sterilization by observing if contaminates appear. Discard all broths which contain growths. The uncontaminated jars are now ready for inoculation. Spray the room and clean the work area as described under pure culture techniques. Also spray the outside parts of the stock dishes and culture jars. Lift the lid of a stock dish just enough and pick up a fragment of mycelium with an inoculation loop that has been flame sterilized and allowed a moment to cool. Lift the cover of the jar, place the mycelium fragment in the broth and cover the jar immediately. Repeat this until all jars have been inoculated. Refrigerate all unused stock cultures. Tighten the jar covers and shake well to disperse the inoculum throughout the broth. This also aerates the medium; the mycelium needs oxygen for life support and growth. Loosen the lids again and place the jars on the growing shelf for 10-12 days at 70-75 degrees F. If other species than Psilocybe cubensis are used, adjust the temperature accordingly. Every 2-3 days tighten the jar covers, shake to aerate and disperse mycelium, reloosen the covers, and return the jars to the shelf.
The process of growth can be followed with a saccharimeter. The maximum growth and highest percentage of psilocybin occurs about four days after all of the broth's sugar content has been used up. The mycelium should be harvested at this time. Any jars that cannot be harvested on that day should be refrigerated until this can be done.

HARVESTING AND DRYING

Filter the medium of each jar through a clean flannel cloth, collect the mycelial material from the cloth, and place it in a pyrex baking dish. Do the same with each jar of mycelium until each baking dish is about 1/3 full with mycelia. Dry these in an oven at no higher temperature than 200 deg F. Use an oven thermometer. Do not rely upon the temperature indications on the oven knob as these may vary from accuracy. Check the baking dishes periodically. When the material first appears to have dried shut off the heat and let the dishes stay in the oven until it has cooled. This ensures the evaporation of residual moisture. Each cultivation jar should yield 50-100 grams of wet mycelium. Fresh mycelium contains about 90 percent water, so this much would dry down to 5 to 10 grams of crumbly material. Each baking dish would contain a dozen or more mycelia.

EXTRACTION

Crumble and pulverize the dried mycelial material and combine each 100 mg of this material with 10 ml of methanol. Place the flask in a hot water bath for four hours. Filter the liquids with suction through a filter paper in a buchner funnel with Celite to prevent clogging. Collect and save the filtrate liquids. Heat the slurry (the mush in the filter paper) two more times in methanol as before, filter, and accumulate the liquids of the three extractions. To be certain that all of the alkaloids have been extracted do a small extraction with a portion of the used slurry and test with Keller's reagent (glacial acetic acid, ferrous chloride, and concentrated sulfuric acid). If there is a violet indication, alkaloids are still present and further extraction is in order. In an open beaker evaporate the liquids to total dryness with a hot water bath or by applying a hair dryer. Be certain that all traces of methanol have been removed. The remaining residue should contain 25-50 percent psilocybin/psilocin mixture. Greater purification can be achieved, but would require other solvents and chromatography equipment and is hardly necessary.
Each 100 grams of dried mycelium should yield about 2 grams of extracted material. This should contain at least 500 mg of psilocybin/psilocin mixed or about fifty 10 mg doses. Theoretically psilocin should have the same effect upon the user as psilocybin. The only difference between the two is that the later has a phosphate bond which disappears immediately after assimilation in the body. In other words, in the body psilocybin turns into psilocin. Psilocybin is a fairly stable compound, but psilocin is very susceptible to oxidization. It is best to keep the extracted material in a dry air tight container under refrigeration. A sack of silica-gel can be placed in the container to capture any moisture that may enter.

DOSAGE

The standard dose of psilocybin or psilocin for a 150 lb person is a 6-20 mg dose. We will figure the average dose as 10 mg. The crude alkaloid extraction process given here yields a brownish crystalline powder that is at least 25 percent pure. Each mason jar should contain at least 50 grams of wet mycelium. After drying this would be about 5 grams of material. The crude material extracted from this should contain 25-30 mg of psilocybin/ psilocin or roughly 2-3 hits. This yield may very to some extent depending upon several factors. Many of these species contain less of these alkaloids than dose Psilocybe cubensis and the alkaloidal content of this species may very in different strains. Cultivation conditions have alot to do with yield too. Higher temperatures (75 degrees F.) cause more rapid growth but lesser psilocybin content than do lower temperatures (70 degrees F.) One must test each new batch of extracted material to determine the proper distribution of dosages. Depending on the potency of the mycelia and how well the extraction was conducted the dose may range between 25 and 100 mg. Also bear in mind that the dose varies for different individuals.

LARGE SCALE PRODUCTION

The techniques and procedures described in this guide can be employed to cultivate modest supplies of psilocybin for personal use, or they can be expanded to apply to the large scale production of many thousand doses per week. A small 10 x 15 foot room with standard 8 foot ceilings can be set up to produce an unending yield of at least 5000 doses per week. The stock culture shelves here are 1 foot deep and 5 feet long. Each could hold twenty 15 cm petri dishes. If shelving is spaced six inches apart, there can be as many as 16 shelves stacked in this space. This would allow for up to 300 stock culture dishes going at one time. The crop culture shelves can be stacked ten inches apart, accommodating one quart size mason jars and giving ten levels. With the dimensions of the room as mentioned this much shelving could hold about 2800 jars (3 deep and 3 per running foot). The entire room - walls, ceiling, and shelving - should be painted with a white, glossy kitchen enamel. This is not only an important sanitary measure, but also improves the efficiency and even distribution of light in the room. Lighting should be provide by a few banks of wide spectrum fluorescent tubes fairly evenly distributed across the ceiling and turned on for 10-12 hours regularly each day. These are great dust catchers, however, and must be wiped clean periodically. The work table should also be painted with a hard smooth, white finish. If the table is metal, a small, clean cutting board must be provided on which to pin down mushroom caps when disecting them. Shelf boards on the wall next to the table may be extended above the table to provide space for storage of work equipment and ready containers. A hood should be constructed around the table to protect it from dust, etc. A fume hood with a flu vent and spark-free exhaust fan should be constructed over the extraction area to remove toxic and combustible methanol vapors.
Extraction is preferably conducted in another room. If the cultivation room is used for extraction while the cultures are growing, care must be taken that the heat from the extraction process does not alter the room temper- ature. The fume hood will help by carrying off most of the heat. A vinyl shower curtain should be hung around the work table to shield the area of breezes when anyone enters or exits the room. Another vinyl curtain can be hung just inside the entrance to serve as a dust trap. A person entering the room would close the door behind him before pulling the curtain aside - and vice versa on exiting. The floor should be white vinyl or asphalt tile or painted white and coated with verathane or polyurethane. There should be no cloth or carpeting in the room except for a supply of clean worl clothing and surgical masks. The only other items in the room would be a stool at the work table, a three step ladder for reaching the upper shelves, and a small table on rollers on which to place jars and dishes when making the rounds of the shelves.
Unless one has a large staff of assistants it would be impossible to inoculate 2800 jars in one work session. After getting used to the work one could do about 100 jars an hour. The best procedure is to set up a continuous rotation of inoculations. Working about three hours a day about 235 jars could be inoculated each session. All 2800 jars could be inoculated in 12 days. Sections of shelving would be divided into groups of 235 jars, and these sections would be labled with the date and approximate time of inoculation. The work schedule for cultivation would be as follows:
DAYINOCULATESHAKEHARVESTREINOCULATE
MonGroup A
TueGroup B
WedGroup CGroup A
ThuGroup DGroup B
FriGroup EGroups A & C
SatGroup FGroups B & D
SunGroup GGroups A, C, & E
MonGroup HGroups B, D, & F
TueGroup IGroups A, C, E, & G
WedGroup JGroups B, D, F, & H
ThuGroup KGroups A, C, E, G, & I
FriGroup LGroups B, D, F, H, & J
SatCommence Reinoculation (See Col. 5)Groups C, E, G, I, & KGroup AGroup A-2
Sun
Groups D, F, H, J, & LGroup BGroup B-2
Mon
Groups E, G, I, K, & A-2Group CGroup C-2
Tue
Groups F, H, J, L, & B-2Group DGroup D-2
etc.
etc.etc.etc.
This would represent the first 2 weeks of the continuous cultivation cycle. The continuation of this schedule is obvious: shaking every other day, harvesting approximately every 12 days, and resterilizing, refilling with fresh medium, autoclaving, and reinoculating the jars liberated by the days harvest. If the total number of jars is 2800, each group would consist of 235 jars. The same schedule could, of course, be adapted to any number of jars. Drying of the mycelia should be done within a few hours after harvesting. Otherwise enzymes in the material will begin to destroy the active alkaloids. Once dried the material can be stored in cool, dark, dry place until enough daily harvests have been accumulated to do an extraction. If the mycelia can not be dried right away it can be kept in a refrigerator for a day or two, or longer times in a freezer.

MAINTAINING A PERPETUAL PSILOCYBIN FARM

Fresh inoculum can come from stock culture dishes kept under refrigeration. If these should become depleted, healthy strains of mycelium from the crop cultures can be used to inoculate sterilized agar media in dishes. To do so shake the crop culture jar violently to break up the mycelium. Then transfer drops of the liquid into autoclaved petri dishes of unused agar medium with a sterilized pipette and let it grow as before. If this reinoculation of stock cultures from existing crops is continued over a long period of time, the strain will eventually weaken due to what is known as the senescence factor. To avoid this alternate the media used in the stock dishes. That is: if PDA is used the first time, use MEA the second time and PDA again the next time, etc.

RECOMMENDED READING

If you cannot find these books in a bookstore, they can be ordered by mail from their publishers:
  • HOMEGROWN HIGHS
    M.J. Superweed, 1972. High potency cultivation techniques for several psychoactive plants including peyote, San Pedro, coleus, and morning glory; plus a special medium formula and practical method for maximum mcycelial growth and extra-high psilocybin yield. The formula can be used in combination with the large-scale production methods in our guide. Send $1.50 plus .50 handling to: Flash Mail Order Post Express, 9926 Haldeman Ave, Suite 3B, Philadelphia, Pa. 19115 PSILOCYBIN - MAGIC MUSHROOM GROWERS GUIDE.
    O.T. Oss and O.N. Oeric, 1976. Excellent guide for those who wish to cultivate carpophores of Stropharia Cubensis. Nicely illustrated with black and white and color photographs. [ The photos on this web page were scanned in from O&E -- SDIZ ] Send $4.95 plus .50 handling to: Flash Mail Order Post Express, 9926 Haldeman Ave, Suite 3B, Philadelphia, Pa. 19115.
Two other highly recommended books - Keys to Genera of Higher Fungi and Poisonous and Hallucinogenic Mushrooms are discussed earlier in this guide.
http://www.lycaeum.org/~sputnik/